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Meera (Toronto, On, Canada) on 07/06/2011

Could someone please provide a single, lucid description of how to dilute and consume Methylene Blue? The concentration of the stuff sold at pet stores is "1, 943mg per 100ml. " How should this standard, bought solution be diluted, according to metric and Imperial systems of measurement? Measurements using cubic centimetres and the like are not much good to those of us without chemistry labs. ) Thanks.
REPLY   2      

Replied By David (Vicksburg, Ms, Usa) on 08/10/2011

1 cubic centimeter = 1 mL

1 mL = 1 g

The above are temperature dependent but work well for normal laboratory or home use. The concentration of your stock solution is 19,430 mg/L. MB solubility is approx. 1 g per 25 mL of water, 65 mL of alcohol.

At maximum solubility the concentration is 40,000 mg/L so your stock solution is a little less than 50% by volume.

Dilutions can be calculated by ratio and proportion using this formula

M1*V1=M2*V2

where M1 is the mass or concentration of stock solution. M2 is the mass or concentration of target solution. V1 is the volume of stock solution required to make target solution.

V2 is the volume you want of the target solution. for example to make 100 mL of a 0. 1% solution

50*V1 = 0.1* 100 so

V1 = 10/50

V1 = 0. 2 mL or 0. 2 grams of the stock solution

I hope this help you understand how dilutions work. I do not know what concentrations you should injest. My example is just to show you how do make the dilutions.

REPLY   4      

Replied By Flora (Calgary, Alberta, Canada) on 11/28/2012

hi Meera ; you can purchase MB at Script pharmacy in Calgary Alberta. I am sure you have compounding pharmacys in TO. Script made mine to the concentrarion I asked for! This should make it easier for you. Hope that helps. Flora
REPLY   1      

Replied By Lawrence (toronto) on 04/18/2022

I have tried 2 compounding pharmacies in Toronto- they say need prescription. If anyone has a compounding pharmacy that will make the 1% solution, I can send you money to buy for me....thnx

Replied By Bob (San Francisco) on 06/23/2016

I use two drops in a shot glass of distilled or spring water.
REPLY   16      

Replied By J. Park (Cerritos, Ca) on 02/19/2017

I know it's way late, I guess you already have answer, but I see so many confusions about % of solution, so here it is for those still confused:

% = g/cc, what you have 1.943g/100cc = 0.019 =~ 2% solution.

To make 0.1%, add 19 x water to it.

But better way of using it is use what Bill said:

. just use 1 drop and consider it to be 20 drops of standard 0.1%.

. add 20oz water to it. Now your 20oz is equiv. to 20 drops.

. If you take 1 oz, you just took 1 drop,

take 2 oz, you just took 2 drops, etc...

Good luck.

REPLY   4